Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP (TCC-HM00VB)

Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 40.6ng/ml determined by ELISA.

The purity of Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Purity: > 95% as determined by HPLC
Activity: Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 40.6ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers

Catalog #TCC-HM00VB

Price

$1,765⁰⁰

Size

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Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP (TCC-HM00VB) Summary

Product Description

Recombinant Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP is expressed from HEK293.It contains NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers.

Product Background

The 1G4c113 TCR is an engineered, affinity-matured variant of the 1G4 T-cell receptor that specifically recognizes the peptide fragment of NY-ESO-1 (SLLMWITQC) presented on the HLA-A*02:01. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.

Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP (TCC-HM00VB) Specifications/Details

Protein	NY-ESO-1 TCR&CD3
Synonyms	NY-ESO-1 TCR&CD3;NY-ESO1TCR&CD3
Species	Human
Biotinylated	yes
Amino Acid Range	NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Activity	Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 40.6ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 300mM L-arginine (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 40.6ng/ml determined by ELISA.
	Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 7.7ng/ml determined by ELISA.
	The purity of Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP is greater than 95% as determined by SEC-HPLC.