Regular price
$1,765.00
Order by 4pm ET for delivery on:
Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP (TCC-HM00VB) Summary
Product Description |
Recombinant Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP is expressed from HEK293.It contains NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers. |
Product Background |
The 1G4c113 TCR is an engineered, affinity-matured variant of the 1G4 T-cell receptor that specifically recognizes the peptide fragment of NY-ESO-1 (SLLMWITQC) presented on the HLA-A*02:01. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface. |
Product Category |
Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / VLP |
Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP (TCC-HM00VB) Specifications/Details
Protein |
NY-ESO-1 TCR&CD3 |
Synonyms |
NY-ESO-1 TCR&CD3 |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers |
Activity |
Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 40.6ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 300mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 40.6ng/ml determined by ELISA. |
|
|
Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP at 5ug/mL (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 7.7ng/ml determined by ELISA. |
|
|
The purity of Biotinylated Human NY-ESO-1 TCR&CD3 Complex VLP is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
