Biotinylated Human Oncostatin M/OSM Protein (ONM-HM401B)
Catalog: ONM-HM401B
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| Product Description | Recombinant Biotinylated Human Oncostatin M/OSM Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Arg221. |
Product Background |
OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. |
Product Category |
Recombinant Protein |
Target |
Oncostatin M/OSM |
| Synonyms | Oncostatin M; OSM; MGC20461 |
Gene/Protein ID |
P13725 |
Species |
Human |
Exact Sequence |
Ala26-Arg221 |
| Molecular Weight | The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 0.94 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human LIF R, His Tag immobilized on CM5 Chip can bind Biotinylated Human Oncostatin M, His Tag with an affinity constant of 0.94 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Oncostatin M is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Oncostatin M on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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