Biotinylated Human OSMR beta Protein (OSM-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human OSMR beta, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu28-Met740
Catalog #OSM-HM401B
Regular price $577.50
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Biotinylated Human OSMR beta Protein (OSM-HM401B) Summary

Product Description

Recombinant Biotinylated Human OSMR beta Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu28-Met740.

Product Background

OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human OSMR beta Protein (OSM-HM401B) Specifications/Details

Protein
OSMR beta
Synonyms
IL-31R subunit beta; IL-31R-beta; IL-31RB; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1 ;IL-31R subunit β; IL-31R-β; OSMR β
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Glu28-Met740
Molecular Weight
The protein has a predicted MW of 84.03 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human OSMR beta, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human OSMR beta, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. Immobilized Biotinylated Human OSMR beta, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
Biotinylated Human OSMR beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human OSMR beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human OSMR beta is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human OSMR beta is greater than 95% as determined by SEC-HPLC.
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