Biotinylated Human OSMR Protein (OSM-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human OSMR, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu28-Met740

Catalog: OSM-HM401B

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Product Description	Recombinant Biotinylated Human OSMR Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu28-Met740.
Product Background	OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.
Product Category	Recombinant Protein

Target	OSMR
Gene/Protein ID	Q99650-1
Species	Human
Exact Sequence	Glu28-Met740
Molecular Weight	The protein has a predicted MW of 84.03 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human OSMR, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human OSMR, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
	The purity of Biotinylated Human OSMR is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human OSMR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.