Biotinylated Human OSMR Protein (OSM-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human OSMR, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu28-Met740
Catalog #OSM-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human OSMR Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu28-Met740.
Product Background
OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.
Product Category
Recombinant Protein
Target
OSMR
Synonyms
IL-31R subunit beta; IL-31R-beta; IL-31RB; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1 ;IL-31R subunit β; IL-31R-β; OSMR β
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Glu28-Met740
Molecular Weight
The protein has a predicted MW of 84.03 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human OSMR, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human OSMR, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
Biotinylated Human OSMR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human OSMR is greater than 95% as determined by SEC-HPLC.

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