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Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) (OXL-HM140B) Summary
Product Description |
Recombinant Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183 trimer design. |
Product Background |
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) (OXL-HM140B) Specifications/Details
Protein |
OX40 Ligand/TNFSF4 |
Synonyms |
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln51-Leu183 |
Molecular Weight |
The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. |
Product Tag |
N-His-Flag |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. |
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Biotinylated Human OX40 Ligand Trimer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 90%. |
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The purity of Biotinylated Human OX40 Ligand Trimer is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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