Name: Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) (OXL-HM140B)
Brand: KactusBio
SKU: OXL-HM140B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) (OXL-HM140B)

Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA.

Biotinylated Human OX40 Ligand Trimer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 90%.

The purity of Biotinylated Human OX40 Ligand Trimer is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln51-Leu183

Catalog #OXL-HM140B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183.
Product Background	Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	OX40 Ligand/TNFSF4
Synonyms	OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34
Accession	P23510-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gln51-Leu183
Molecular Weight	The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-160 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA.
	Biotinylated Human OX40 Ligand Trimer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 90%.
	The purity of Biotinylated Human OX40 Ligand Trimer is greater than 90% as determined by SEC-HPLC.