Name: Biotinylated Human OX40/TNFRSF4/CD134 Protein (OX4-HM440B)
Brand: KactusBio
SKU: OX4-HM440B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human OX40/TNFRSF4/CD134 Protein (OX4-HM440B)

Biotinylated Human OX40, His Tag immobilized on CM5 Chip can bind Human OX40 Ligand, hFc Tag with an affinity constant of 23.48 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).

Biotinylated Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human OX40 is greater than 95% as determined by SEC HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). The affinity constant of 23.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu29-Ala216

Catalog #OX4-HM440B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Ala216.
Product Background	Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	OX40/TNFRSF4/CD134
Synonyms	CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16
Accession	P43489
Species	Human
Biotinylated	yes
Amino Acid Range	Leu29-Ala216
Molecular Weight	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). The affinity constant of 23.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human OX40, His Tag immobilized on CM5 Chip can bind Human OX40 Ligand, hFc Tag with an affinity constant of 23.48 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
	Biotinylated Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human OX40 is greater than 95% as determined by SEC HPLC.