Biotinylated Human P53 R175H (HLA-A*02:01) Complex Protein (MHC-HM415B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-P53 R175H(HLA-A*02:01) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Biotinylated Human P53 R175H (HLA-A*02:01) Complex, His Tag with the EC50 of 0.6μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide

Catalog: MHC-HM415B

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Product Description Recombinant Biotinylated Human P53 R175H (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide.
Product Category Recombinant Protein
Target P53 R175H (HLA-A*02:01) Complex
Synonyms MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide
Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-P53 R175H(HLA-A*02:01) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Biotinylated Human P53 R175H (HLA-A*02:01) Complex, His Tag with the EC50 of 0.6μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in 20mM PB, 0.5M NaCl (pH 8.0). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized Anti-P53 R175H(HLA-A*02:01) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Biotinylated Human P53 R175H (HLA-A*02:01) Complex, His Tag with the EC50 of 0.6μg/ml determined by ELISA.
The purity of Biotinylated Human P53 R175H (HLA-A*02:01) Complex was greater than 95% as determined by SEC-HPLC.
Biotinylated Human P53 R175H (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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