• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LDLR, hFc Tag (Cat. LDL-HM201) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PCSK9, His Avi Tag with the EC50 of 17.3ng/ml determined by ELISA.
  • Amino Acid Range: Gln31-Gln692
Catalog #PCS-HM490B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human PCSK9 Protein (PCS-HM490B) Summary

Product Description

 Recombinant Biotinylated Human PCSK9 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gln31-Gln692.

Product Background

 Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.

Biotinylated Human PCSK9 Protein (PCS-HM490B) Specifications/Details

Protein
 PCSK9
Synonyms
 PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1;NARC1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln31-Gln692
Molecular Weight
 The protein has a predicted MW of 73.95 kDa. Due to autocatalytic cleavage, the protein migrates to 15-17 kDa (pro-peptide) and 65-85 kDa (mature chain) based on Bis-Tris PAGE result.
Activity
 Immobilized Human LDLR, hFc Tag (Cat. LDL-HM201) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PCSK9, His Avi Tag with the EC50 of 17.3ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LDLR, hFc Tag (Cat. LDL-HM201) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PCSK9, His Avi Tag with the EC50 of 17.3ng/ml determined by ELISA. Immobilized Human LDLR, hFc Tag (Cat. LDL-HM201) at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PCSK9, His Avi Tag with the EC50 of 17.3ng/ml determined by ELISA.
Immobilized Biotinylated Human PCSK9, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-PCSK9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. Immobilized Biotinylated Human PCSK9, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-PCSK9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
Biotinylated Human PCSK9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human PCSK9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human PCSK9 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human PCSK9 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...