Biotinylated Human PD-1/PDCD1 Protein (PD1-HM501B)
Biotinylated Human PD-1/PDCD1 Protein (PD1-HM501B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu25-Gln167
Catalog #PD1-HM501B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Leu25-Gln167.

Product Background

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

PD-1/PDCD1

Synonyms

PDCD1; PD1; CD279; SLEB2; PD-1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Leu25-Gln167

Molecular Weight

The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.23ug/ml determined by ELISA. Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.23ug/ml determined by ELISA.
Biotinylated Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%. Biotinylated Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.
The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.

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