Biotinylated Human PD-1/PDCD1 Protein (PD1-HM501B)
Catalog: PD1-HM501B
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| Product Description | Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal.It contains Leu25-Gln167. |
| Product Category | Recombinant Protein |
| Target | PD-1/PDCD1 |
| Synonyms | PDCD1; PD1; CD279; SLEB2; PD-1 |
| Gene/Protein ID | Q15116-1 |
| Species | Human |
| Exact Sequence | Leu25-Gln167 |
| Molecular Weight | The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human PD-L1, mFc tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.19μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human PD-L1, mFc tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.19μg/ml determined by ELISA. |
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The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%. |
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