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Biotinylated Human PD-1/PDCD1 Protein, Ultra Low Endotoxin (PD1-HM501B-UL) Summary
Product Description |
Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu25-Gln167. |
Product Background |
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human PD-1/PDCD1 Protein, Ultra Low Endotoxin (PD1-HM501B-UL) Specifications/Details
Protein |
PD-1/PDCD1 |
Synonyms |
PDCD1; PD1; CD279; SLEB2; PD-1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu25-Gln167 |
Molecular Weight |
The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.45ug/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1)-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.45ug/ml determined by ELISA. |
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Biotinylated Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%. |
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Serial dilutions of Anti-PD-L1 Antibody were added into Human PD-L1, His Tag : Biotinylated Human PD-1, hFc Avi Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 11.7ng/ml. |
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The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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