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Biotinylated Human PD-L1/B7-H1 Protein, Ultra Low Endotoxin (PDL-HM410B-UL) Summary
Product Description |
Recombinant Biotinylated Human PD-L1/B7-H1 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Arg238. |
Product Background |
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins | ADC targets |
Biotinylated Human PD-L1/B7-H1 Protein, Ultra Low Endotoxin (PDL-HM410B-UL) Specifications/Details
Protein |
PD-L1/B7-H1 |
Synonyms |
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe19-Arg238 |
Molecular Weight |
The protein has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human PD-L1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human PD-1, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human PD-L1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human PD-1, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). |
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Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 9.4ng/ml determined by ELISA. |
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Biotinylated Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-PD-1 Antibody were added into Human PD-1, hFc Tag: Biotinylated Human PD-L1, His Avi Tag binding reactions. The half maximal inhibitiory concentration (IC50) is 0.22ug/ml. |
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Loaded Biotinylated Human PD-L1, His Tag on Streptavidin-Biosensor can bind Human PD-1, His Tag with an affinity constant of 0.94 µM as determined in BLI assay . |
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The purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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