Biotinylated Human PD-L2/B7-DC Protein (PDL-HM402B)
Catalog: PDL-HM402B
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| Product Description | Recombinant Biotinylated Human PD-L2/B7-DC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Thr220. |
Product Background |
PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals. |
Product Category |
Recombinant Protein |
Target |
PD-L2/B7-DC |
| Synonyms | PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc |
Gene/Protein ID |
Q9BQ51-1 |
Species |
Human |
Exact Sequence |
Leu20-Thr220 |
| Molecular Weight | The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human PD-L2 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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