Biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40AB)
Catalog: PFR-HM40AB
Order now get it on
| Product Description | Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524. |
Product Background |
Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF. |
Product Category |
Recombinant Protein |
Target |
PDGF R alpha/PDGFRA |
| Synonyms | PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2 |
Gene/Protein ID |
P16234 |
Species |
Human |
Exact Sequence |
Gln24-Glu524 |
| Molecular Weight | The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-PDGF R alpha Antibody, hFc Tag T at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PDGF R alpha, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Anti-PDGF R alpha Antibody, hFc Tag T at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PDGF R alpha, His Tag with the EC50 of 0.16ug/ml determined by ELISA. |
|
|
The purity of Biotinylated Human PDGF R alpha is greater than 95% as determined by SEC-HPLC. |
|
|
Biotinylated Human PDGF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products