Biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40AB)
|Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524.
|Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
|PDGF R alpha/PDGFRA
|PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2
|The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result.
|Immobilized Anti-PDGF R alpha Antibody, hFc Tag T at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PDGF R alpha, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.