Biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40AB)
Biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40AB)
Biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40AB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human PDGF R alpha, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln24-Glu524
Catalog #PFR-HM40AB
Regular price $577.50
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Product Description

Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524.

Product Background

Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
PDGF R alpha/PDGFRA
Synonyms
PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln24-Glu524
Molecular Weight
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human PDGF R alpha, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human PDGF R alpha, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human PDGF R alpha, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).
Biotinylated Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human PDGF R alpha is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human PDGF R alpha is greater than 95% as determined by SEC-HPLC.
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