Name: Biotinylated Human PDGF R beta/CD140b Protein (PGF-HM4RBB)
Brand: KactusBio
SKU: PGF-HM4RBB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human PDGF R beta/CD140b Protein (PGF-HM4RBB)

Immobilized Anti-PDGF R beta Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PDGF R beta, His Tag with the EC50 of 12.5ng/ml determined by ELISA.

Biotinylated Human PDGF R beta on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human PDGF R beta is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-PDGF R beta Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PDGF R beta, His Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu33-Phe530

Catalog #PGF-HM4RBB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human PDGF R beta/CD140b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu33-Phe530.
Product Background	PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
Product Category	Recombinant Protein / Antibody discovery

Protein	PDGF R beta/CD140b
Synonyms	JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-β; PDGF R β
Accession	P09619
Species	Human
Biotinylated	yes
Amino Acid Range	Leu33-Phe530
Molecular Weight	The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-PDGF R beta Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PDGF R beta, His Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-PDGF R beta Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PDGF R beta, His Tag with the EC50 of 12.5ng/ml determined by ELISA.
	Biotinylated Human PDGF R beta on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human PDGF R beta is greater than 95% as determined by SEC-HPLC.