Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer Protein (MHC-HM53RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*33:03) & Ile21-Met119(B2M)
Catalog #MHC-HM53RB
Regular price $310.00
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Product Description

Recombinant Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*33:03) and Ile21-Met119(B2M).

Product Background

HLA-A*33:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*33:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

Peptide Ready HLA-A*33:03&B2M

Synonyms

MHC; HLA-A*33:03; Peptide Ready

MHC Form

Monomer

Allele

HLA-A*33:03

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly25-Thr305(HLA-A*33:03) & Ile21-Met119(B2M)

Molecular Weight

The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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