Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer Protein (MHC-HM53RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*33:03) & Ile21-Met119(B2M)
Catalog #MHC-HM53RB
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Product Description
 Recombinant Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*33:03) and Ile21-Met119(B2M).
Product Background
 HLA-A*33:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*33:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category
 Recombinant Protein
Target
 Peptide Ready HLA-A*33:03&B2M
Synonyms
 MHC; HLA-A*33:03; Peptide Ready
MHC Form
 Monomer
Allele
 HLA-A*33:03
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*33:03) & Ile21-Met119(B2M)
Molecular Weight
 The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer is greater than 90% as determined by SEC-HPLC.

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