Name: Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer Protein (MHC-HM62RB)
Brand: KactusBio
SKU: MHC-HM62RB-100UG
Price: 341.0 USD
Availability: InStock

Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer Protein (MHC-HM62RB)

Immobilized Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.

Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
Amino Acid Range: Gly25-Pro300(HLA-B*27:05) & Ile21-Met119(B2M)

Catalog #MHC-HM62RB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer Protein (MHC-HM62RB) Summary

Product Description	Recombinant Biotinylated Human Peptide Ready HLA-B27:05&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Pro300(HLA-B27:05) & Ile21-Met119(B2M).
Product Background	HLA-B27:05&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-B27:05. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs

Peptide Ready HLA-B*27:05&B2M Specifications

Protein	Peptide Ready HLA-B*27:05&B2M
Synonyms	MHC; HLA-B*27:05; Peptide Ready
MHC Form	Monomer
Allele	HLA-B*27:05
Accession	AAA36221.1(HLA-B*27:05)&P61769(B2M)
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Pro300(HLA-B*27:05) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 100mM Arginine (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
	Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Peptide Ready HLA-B*27:05&B2M Monomer is greater than 95% as determined by SEC-HPLC.