SEC-HPLC chromatogram showing a main protein peak at 9.4 minutes with a minor peak at 8.4 minutes, used for purity and aggregate assessment.
Biotinylated Human Peptide Ready HLA-G&B2M Monomer-Protein (MHC-HM45RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-G) & Ile21-Met119(B2M)
Catalog #MHC-HM45RB
Regular price $341.00
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Biotinylated Human Peptide Ready HLA-G&B2M Monomer-Protein (MHC-HM45RB) Summary

Product Description

Recombinant Biotinylated Human Peptide Ready HLA-G&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-G) and Ile21-Met119(B2M).

Product Background

Peptide Ready HLA-G&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-G. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs

Peptide Ready HLA-G&B2M Specifications

Protein
Peptide Ready HLA-G&B2M
Synonyms
MHC; HLA-G; Peptide Ready

MHC Form

Monomer

Allele

HLA-G
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-G) & Ile21-Met119(B2M)
Molecular Weight
The protein has a predicted MW of 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human Peptide Ready HLA-G&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Peptide Ready HLA-G&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Peptide Ready HLA-G&B2M Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Peptide Ready HLA-G&B2M Monomer is greater than 95% as determined by SEC-HPLC.
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