Name: Biotinylated Human PLAU/uPA Protein (active form) (PLA-HM401B)
Brand: KactusBio
SKU: PLA-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human PLAU/uPA Protein (active form) (PLA-HM401B)

Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).

Biotinylated Human PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human PLAU is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser21-Leu431

Catalog #PLA-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-Terminus fragment (Ser21-Lys155).
Product Background	Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category	Recombinant Protein / Antibody discovery

Protein	PLAU/uPA
Synonyms	PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Accession	P00749-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ser21-Leu431
Molecular Weight	The protein has a predicted MW of 49.3 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Bis-Tris PAGE result.
Activity	Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
	Biotinylated Human PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human PLAU is greater than 95% as determined by SEC-HPLC.