Biotinylated Human PLAU/uPA Protein (active form) (PLA-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human uPAR, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser21-Leu431

Catalog: PLA-HM401B

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Product Description Recombinant Biotinylated Human PLAU/uPA Protein (active form) is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-terminal fragment (Ser21-Lys155).
Product Category Recombinant Protein
Target PLAU/uPA
Synonyms PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Gene/Protein ID P00749-1
Species Human
Exact Sequence Ser21-Leu431
Molecular Weight The protein has a predicted MW of 49.3 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa (long chain A), 35-38 kDa (chain B) and 52-60 kDa (long chain A&chain B) based on Tris-Bis PAGE result.
Activity Immobilized Human uPAR, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with blue ice or dry ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human uPAR, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.6ng/ml determined by ELISA.
The purity of Biotinylated Human PLAU/uPA (activated by trypsin) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human PLAU/uPA (activated by trypsin) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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