Name: Biotinylated Human PLAU/uPA Protein (active form) (PLA-HM401B)
Brand: KactusBio
SKU: PLA-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human PLAU/uPA Protein (active form) (PLA-HM401B)

Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).

Biotinylated Human PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human PLAU is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser21-Leu431

Catalog #PLA-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-Terminus fragment (Ser21-Lys155).
Product Background	Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	PLAU/uPA
Synonyms	PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Accession	P00749-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ser21-Leu431
Molecular Weight	The protein has a predicted MW of 49.3 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa, 35-38 kDa and 52-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human uPAR, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
	Biotinylated Human PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human PLAU is greater than 95% as determined by SEC-HPLC.

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