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Biotinylated Human PSGL-1 Protein (PSG-HM462B) Summary
Product Description |
Recombinant Biotinylated Human PSGL-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gln42-Cys320. |
Product Background |
P-selectin glycoprotein ligand-1 (PSGL-1) has long been studied as an adhesion molecule involved in immune cell trafficking and is recognized as a regulator of many facets of immune responses by myeloid cells. PSGL-1 also regulates T cell migration during homeostasis and inflammatory settings. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human PSGL-1 Protein (PSG-HM462B) Specifications/Details
Protein |
PSGL-1 |
Synonyms |
CLA; PSGL-1; PSGL1; CD162; SELPLG; Selectin P ligand; Selp1; Selpl |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln42-Cys320 |
Molecular Weight |
The protein has a predicted MW of 31.74 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Biotinylated Human PSGL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human PSGL-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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