Biotinylated Human RGM-C Protein (RGM-HM60CB)
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human RGM-C, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln36-Asp400
Catalog #RGM-HM60CB
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Product Description
 Recombinant Biotinylated Human RGM-C Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Gln36-Asp400.
Product Background
 RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
Product Category
 Recombinant Protein
Target
 RGM-C
Synonyms
 Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln36-Asp400
Molecular Weight
 The protein has a predicted MW of 40 kDa (mature), 26 kDa (C-terminus peptide) and 14 kDa (N-terminus peptide). Due to furin cleavage site, the protein migrates to 55-60 kDa, 30-40 kDa, 20-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human RGM-C, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human RGM-C, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Biotinylated Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human RGM-C is greater than 95% as determined by SEC-HPLC.

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