Biotinylated Human RGM-C Protein (RGM-HM60CB)
Biotinylated Human RGM-C Protein (RGM-HM60CB)
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human RGM-C, Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln36-Asp400
Catalog #RGM-HM60CB
Regular price $600.00
/
Size

Order now, ships by:

Product Description

Recombinant Biotinylated Human RGM-C Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Gln36-Asp400.

Product Background

RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.

Product Category

Recombinant Protein / Antibody discovery

Protein

RGM-C

Synonyms

Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gln36-Asp400

Molecular Weight

The protein has a predicted MW of 40 kDa (mature), 26 kDa (C-terminus peptide) and 14 kDa (N-terminus peptide). Due to furin cleavage site, the protein migrates to 55-60 kDa, 30-40 kDa, 20-25 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human RGM-C, Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human RGM-C, Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human RGM-C, Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
Biotinylated Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human RGM-C is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human RGM-C is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed