- Express System: HEK293
- Product Tag: C-Avi
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Biotinylated Human RGM-C, Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Gln36-Asp400
Product Description |
Recombinant Biotinylated Human RGM-C Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Gln36-Asp400. |
Product Background |
RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
RGM-C |
Synonyms |
Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln36-Asp400 |
Molecular Weight |
The protein has a predicted MW of 40 kDa (mature), 26 kDa (C-terminus peptide) and 14 kDa (N-terminus peptide). Due to furin cleavage site, the protein migrates to 55-60 kDa, 30-40 kDa, 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human RGM-C, Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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