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Biotinylated Human RGMa Protein (RGM-HM401B) Summary
Product Description |
Recombinant Biotinylated Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys48-Gly422. |
Product Background |
Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human RGMa Protein (RGM-HM401B) Specifications/Details
Protein |
RGMa |
Synonyms |
RGMA; RGM |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Cys48-Gly422 |
Molecular Weight |
The protein has a predicted MW of 44.5 kDa. Due to autocatalytic cleavage, the protein migrates to 27-35 kDa and 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. |
|
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Biotinylated Human RGMa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human RGMa is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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