Biotinylated Human RGMa Protein (RGM-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Cys48-Gly422

Catalog: RGM-HM401B

$525.00
Size:

Order now get it on

Product Description Recombinant Biotinylated Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys48-Gly422.

Product Background

Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS).

Product Category

Recombinant Protein

Target

RGMa
Synonyms RGMA; RGM

Gene/Protein ID

Q96B86-1

Species

Human

Exact Sequence

Cys48-Gly422
Molecular Weight The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.
The purity of Biotinylated Human RGMa is greater than 95% as determined by SEC-HPLC.
Biotinylated Human RGMa on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in