Name: Biotinylated Human RGMa Protein (RGM-HM401B)
Brand: KactusBio
SKU: RGM-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human RGMa Protein (RGM-HM401B)

Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.

Biotinylated Human RGMa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human RGMa is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Cys48-Gly422

Catalog #RGM-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys48-Gly422.
Product Background	Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS).
Product Category	Recombinant Protein

Protein	RGMa
Synonyms	RGMA; RGM
Accession	Q96B86-1
Species	Human
Biotinylated	yes
Amino Acid Range	Cys48-Gly422
Molecular Weight	The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human RGMa, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
	Biotinylated Human RGMa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human RGMa is greater than 95% as determined by SEC-HPLC.