Biotinylated Human Semaphorin 4A/SEMA4A Protein (SEM-HM44AB)

Human LILRB2, hFc Tag (Cat. LIL-HM2B2) captured on CM5 Chip via Protein A can bind Biotinylated Human Semaphorin 4A, His Tag with an affinity constant of 34.05 nM as determined in SPR assay (Biacore T200).

Biotinylated Human Semaphorin 4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Semaphorin 4A is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 34.05 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly33-His683

Catalog #SEM-HM44AB

Price

$578⁰⁰

Size

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Biotinylated Human Semaphorin 4A/SEMA4A Protein (SEM-HM44AB) Summary

Product Description

Recombinant Biotinylated Human Semaphorin 4A/SEMA4A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly33-His683.

Product Background

Neuroimmune semaphorin 4A (Sema4A), a member of semaphorin family of transmembrane and secreted proteins, is an important regulator of neuronal and immune functions. In the nervous system, Sema4A primarily regulates the functional activity of neurons serving as an axon guidance molecule. In the immune system, Sema4A regulates immune cell activation and function, instructing a fine tuning of the immune response.

Biotinylated Human Semaphorin 4A/SEMA4A Protein (SEM-HM44AB) Specifications/Details

Protein	Semaphorin 4A/SEMA4A
Synonyms	Semaphorin-4A; Semaphorin-B; SEMA4A
Accession	Q9H3S1-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly33-His683
Molecular Weight	The protein has a predicted MW of 75.31 kDa. Due to glycosylation, the protein migrates to 85-95 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 34.05 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human LILRB2, hFc Tag (Cat. LIL-HM2B2) captured on CM5 Chip via Protein A can bind Biotinylated Human Semaphorin 4A, His Tag with an affinity constant of 34.05 nM as determined in SPR assay (Biacore T200).
	Biotinylated Human Semaphorin 4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Semaphorin 4A is greater than 95% as determined by SEC-HPLC.