Biotinylated Human SEZ6 Protein (SEZ-HM406B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu20-His925
Catalog #SEZ-HM406B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human SEZ6 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-His925.
Product Background
Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
Product Category
Recombinant Protein
Target
SEZ6
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu20-His925
Molecular Weight
The protein has a predicted MW of 100.7 kDa. Due to glycosylation, the protein migrates to 170-200 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
Biotinylated Human SEZ6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SEZ6 is greater than 95% as determined by SEC-HPLC.

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