Biotinylated Human Siglec-10 Protein (SIG-HM410B)
Catalog: SIG-HM410B
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| Product Description | Recombinant Biotinylated Human Siglec-10 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546. |
Product Background |
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
Product Category |
Recombinant Protein |
Target |
Siglec-10 |
| Synonyms | SLG2; SIGLEC10; MGC126774; PRO940 |
Gene/Protein ID |
Q96LC7-1 |
Species |
Human |
Exact Sequence |
Met17-Thr546 |
| Molecular Weight | The protein has a predicted MW of 61.13 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human Siglec-10, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 81.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human Siglec-10, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 81.3ng/ml determined by ELISA. |
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The purity of Biotinylated Human Siglec-10 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Siglec-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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