Biotinylated Human Siglec-10 Protein (SIG-HM510B)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-Siglec-10 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, hFc Tag with the EC50 of 25ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met17-Thr546

Catalog: SIG-HM510B

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Product Description Recombinant Biotinylated Human Siglec-10 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Met17-Thr546.
Product Category Recombinant Protein
Target Siglec-10
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
Gene/Protein ID Q96LC7-1
Species Human
Exact Sequence Met17-Thr546
Molecular Weight The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-Siglec-10 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, hFc Tag with the EC50 of 25ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-Siglec-10 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, hFc Tag with the EC50 of 25ng/ml determined by ELISA.
The purity of Biotinylated Human Siglec-10 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Siglec-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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