Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-Siglec-10 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 61.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met17-Thr546

Catalog: SIG-HM411B

$657.00
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Product Description Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Met17-Thr546.
Product Category Recombinant Protein
Target Siglec-10 (R119A)
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
Gene/Protein ID Q96LC7-1
Species Human
Exact Sequence Met17-Thr546
Molecular Weight The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-Siglec-10 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 61.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tirs 150mM NaCl, 200mM L-Arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-Siglec-10 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 61.7ng/ml determined by ELISA.
The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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