Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met17-Thr546

Catalog: SIG-HM411B

$525.00
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Product Description Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Product Background

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Product Category

Recombinant Protein

Target

Siglec-10 (R119A)
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
Accession

Species

Human

Exact Sequence

Met17-Thr546
Molecular Weight The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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