Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Catalog: SIG-HM411B
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| Product Description | Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546. |
| Product Category | Recombinant Protein |
| Target | Siglec-10 (R119A) |
| Synonyms | SLG2; SIGLEC10; MGC126774; PRO940 |
| Gene/Protein ID | Q96LC7-1 |
| Species | Human |
| Exact Sequence | Met17-Thr546 |
| Molecular Weight | The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. |
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The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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