Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met17-Thr546
Catalog #SIG-HM411B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Product Background

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Siglec-10 (R119A)

Synonyms

SLG2; SIGLEC10; MGC126774; PRO940

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Met17-Thr546

Molecular Weight

The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
Biotinylated Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

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