Name: Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)
Brand: KactusBio
SKU: SIG-HM411B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B)

Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

Biotinylated Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Met17-Thr546

Catalog #SIG-HM411B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.
Product Background	Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Siglec-10 (R119A)
Synonyms	SLG2; SIGLEC10; MGC126774; PRO940
Accession	Q96LC7-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met17-Thr546
Molecular Weight	The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
	Biotinylated Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.