Name: Biotinylated Human Siglec-4a/MAG Protein (SIG-HM44AB)
Brand: KactusBio
SKU: SIG-HM44AB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Siglec-4a/MAG Protein (SIG-HM44AB)

Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA.

Biotinylated Human Siglec-4a on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly20-Pro516

Catalog #SIG-HM44AB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly20-Pro516.
Product Background	Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
Product Category	Recombinant Protein / Antibody discovery

Protein	Siglec-4a/MAG
Synonyms	MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession	P20916
Species	Human
Biotinylated	yes
Amino Acid Range	Gly20-Pro516
Molecular Weight	The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA.
	Biotinylated Human Siglec-4a on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC.