Product Description |
Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly20-Pro516. |
Product Background |
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). |
Product Category |
Recombinant Protein |
Target |
Siglec-4a/MAG |
Synonyms |
MAG; Siglec4a; Siglec-4a; S-MAG; GMA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly20-Pro516 |
Molecular Weight |
The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA. |
|
|
Biotinylated Human Siglec-4a on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC. |
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