Biotinylated Human Siglec-5/CD170 Protein (SIG-HM405B)
Biotinylated Human Siglec-5/CD170 Protein (SIG-HM405B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Glu17-Thr434
Catalog #SIG-HM405B
Regular price $577.50
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Biotinylated Human Siglec-5/CD170 Protein (SIG-HM405B) Summary

Product Description

Recombinant Biotinylated Human Siglec-5/CD170 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu17-Thr434.

Product Background

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Siglec 5 is putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human Siglec-5/CD170 Protein (SIG-HM405B) Specifications/Details

Protein
Siglec-5/CD170
Synonyms
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Glu17-Thr434
Molecular Weight
The protein has a predicted MW of 49.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 350mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human Siglec-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Siglec-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Siglec-5 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Siglec-5 is greater than 95% as determined by SEC-HPLC.
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