Name: Biotinylated Human SIRP alpha V4 Protein (SRP-HM4V4B)
Brand: KactusBio
SKU: SRP-HM4V4B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human SIRP alpha V4 Protein (SRP-HM4V4B)

Immobilized Human CD47, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.5ug/ml determined by ELISA (QC Test).

Biotinylated Human SIRP alpha V4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD47, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.5ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)

Catalog #SRP-HM4V4B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human SIRP alpha V4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H).
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	SIRP alpha V4
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
Accession	P78324 variant 4
Species	Human
Biotinylated	yes
Amino Acid Range	Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)
Molecular Weight	The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD47, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.5ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD47, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.5ug/ml determined by ELISA (QC Test).
	Biotinylated Human SIRP alpha V4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC.