Biotinylated Human SIRP alpha V4 Protein (SRP-HM4V4B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human CD47, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.47μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)

Catalog: SRP-HM4V4B

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Product Description Recombinant Biotinylated Human SIRP alpha V4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H).
Product Category Recombinant Protein
Target SIRP alpha V4
Synonyms CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID P78324 variant 4
Species Human
Exact Sequence Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)
Molecular Weight The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD47, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.47μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD47, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.47μg/ml determined by ELISA.
The purity of Biotinylated Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human SIRP alpha V4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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