Biotinylated Human SIRP alpha V5 Protein (SRP-HM4V5B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human SIRP alpha V5, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.1μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu31-Arg370

Catalog: SRP-HM4V5B

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Product Description Recombinant Biotinylated Human SIRP alpha V5 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Glu31-Arg370. (The difference with SIRP alpha is in amino acid number 12, change from Ser12 to Phe12.)
Product Category Recombinant Protein
Target SIRP alpha V5
Synonyms CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID P78324 variant 5
Species Human
Exact Sequence Glu31-Arg370
Molecular Weight The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human SIRP alpha V5, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.1μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human SIRP alpha V5, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.1μg/ml determined by ELISA.
The purity of Biotinylated Human SIRP alpha V5 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human SIRP alpha V5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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