Name: Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B)
Brand: KactusBio
SKU: SRP-HM4V8B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B)

Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).

Biotinylated Human SIRP alpha V8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human SIRP alpha V8 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)

Catalog #SRP-HM4V8B

Size

100UG

500UG

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Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B) Summary

Product Description	Recombinant Biotinylated Human SIRP alpha V8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A).
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B) Specifications/Details

Protein	SIRP alpha V8
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA; SIRPα
Accession	ATD50864.1
Species	Human
Biotinylated	yes
Amino Acid Range	Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)
Molecular Weight	The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
	Biotinylated Human SIRP alpha V8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human SIRP alpha V8 is greater than 90% as determined by SEC-HPLC.