Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B)
|Recombinant Biotinylated Human SIRP alpha V8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A).
|Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
|SIRP alpha V8
|Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)
|The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.
|Immobilized Human CD47, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V8, His Tag with the EC50 of 0.53ug/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.