Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B)
Catalog: SRP-HM4V8B
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Product Description | Recombinant Biotinylated Human SIRP alpha V8 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A). |
Product Category | Recombinant Protein |
Target | SIRP alpha V8 |
Gene/Protein ID | ATD50864.1 |
Species | Human |
Exact Sequence | Glu31-Arg369(S44L, S50T, I52T, H54R, V57A) |
Molecular Weight | The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human CD47, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V8, His Tag with the EC50 of 0.53ug/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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