Biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40GB)
Biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40GB)
Biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40GB)
Biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40GB)
Biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40GB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human SIRP Gamma, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu29-Pro360
Catalog #SRP-HM40GB
Regular price $525.00
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Product Description
Recombinant Biotinylated Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.
Product Background
Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.
Product Category
Recombinant Protein
Target
SIRP gamma/CD172g
Synonyms
CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Glu29-Pro360
Molecular Weight
The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human SIRP Gamma, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human SIRP Gamma, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA.
Biotinylated Human SIRP gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
The purity of Biotinylated Human SIRP gamma is greater than 95% as determined by SEC-HPLC.

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