Product Description |
Recombinant Biotinylated Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360. |
Product Background |
Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. |
Product Category |
Recombinant Protein |
Target |
SIRP gamma/CD172g |
Synonyms |
CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Glu29-Pro360 |
Molecular Weight |
The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human SIRP Gamma, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human SIRP Gamma, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. |
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Biotinylated Human SIRP gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.. |
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The purity of Biotinylated Human SIRP gamma is greater than 95% as determined by SEC-HPLC. |
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