Product Description |
Recombinant Biotinylated Human SKP1 Protein is expressed from HEK293 His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163. |
Product Background |
The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes. |
Product Category |
Recombinant Protein |
Target |
SKP1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Pro2-Lys163 |
Molecular Weight |
The protein has a predicted MW of 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His,C-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Biotinylated Human SKP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human SKP1 is greater than 95% as determined by SEC-HPLC. |
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