• Express System: HEK293
  • Product Tag: N-His,C-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Pro2-Lys163
Catalog #SKP-HM401B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human SKP1 Protein (SKP-HM401B) Summary

Product Description

 Recombinant Biotinylated Human SKP1 Protein is expressed from HEK293 His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163.

Product Background

 The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.

Biotinylated Human SKP1 Protein (SKP-HM401B) Specifications/Details

Protein
 SKP1
Synonyms
 p19A; OCP-2; OCP-II; SIII; EMC19; OCP2; SKP1A; TCEB1L
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Pro2-Lys163
Molecular Weight
 The protein has a predicted MW of 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
Product Tag
 N-His,C-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human SKP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human SKP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SKP1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human SKP1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...