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Biotinylated Human SLAMF7/CRACC/CD319 Protein (SMF-HM407B) Summary
Product Description |
Recombinant Biotinylated Human SLAMF7/CRACC/CD319 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser23-Met226 |
Product Background |
CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 65-75 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family.Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Biotinylated Human SLAMF7/CRACC/CD319 Protein (SMF-HM407B) Specifications/Details
Protein |
SLAMF7/CRACC/CD319 |
Synonyms |
CD2 subset 1; CRACC; Novel Ly9; CD319; SLAMF7; CS1; 19A; FOAP-12 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser23-Met226 |
Molecular Weight |
The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human SLAMF7, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human SLAMF7, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. |
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Biotinylated Human SLAMF7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human SLAMF7 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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