Name: Biotinylated Human SOST/Sclerostin Protein (SOT-HM401B)
Brand: KactusBio
SKU: SOT-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human SOST/Sclerostin Protein (SOT-HM401B)

Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA.

Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.7ng/ml determined by ELISA (QC Test).

Biotinylated Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln24-Tyr213

Catalog #SOT-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.
Product Background	SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Product Category	Recombinant Protein / Antibody discovery

Protein	SOST/Sclerostin
Synonyms	sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
Accession	Q9BQB4-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gln24-Tyr213
Molecular Weight	The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA.
	Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.