Biotinylated Human SOST/Sclerostin Protein (SOT-HM401B)
Biotinylated Human SOST/Sclerostin Protein (SOT-HM401B)
Biotinylated Human SOST/Sclerostin Protein (SOT-HM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln24-Tyr213
Catalog #SOT-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.
Product Background
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Product Category
Recombinant Protein
Target
SOST/Sclerostin
Synonyms
sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln24-Tyr213
Molecular Weight
The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Biotinylated Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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