Product Description |
Recombinant Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and LMLGEFLKL peptide. |
Product Background |
Survivin (also known as BIRC5) is an evolutionarily conserved eukaryotic protein that is essential for cell division and can inhibit cell death. Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer |
Synonyms |
Survivin; MHC; MHC I; BIRC5; API4; EPR-1; IAP4; survivin variant 3 alpha |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&LMLGEFLKL |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and LMLGEFLKL peptide |
Molecular Weight |
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). |
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Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer is greater than 95% as determined by SEC-HPLC. |
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