Biotinylated Human Tim-3/HAVCR2 Protein (TIM-HM431B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser22-Arg200

Catalog: TIM-HM431B

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Product Description Recombinant Biotinylated Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Arg200.
Product Category Recombinant Protein
Target Tim-3/HAVCR2
Synonyms CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3
Gene/Protein ID Q8TDQ0-1
Species Human
Exact Sequence Ser22-Arg200
Molecular Weight The protein has a predicted MW of 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 13.2ng/ml determined by ELISA.
The purity of Biotinylated Human Tim-3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Tim-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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