Product Description |
Recombinant Biotinylated Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Arg200. |
Product Background |
Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. |
Product Category |
Recombinant Protein |
Target |
Tim-3/HAVCR2 |
Synonyms |
CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser22-Arg200 |
Molecular Weight |
The protein has a predicted MW of 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Tim-3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 11.3ng/ml determined by ELISA. |
|
|
Biotinylated Human Tim-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Tim-3 is greater than 95% as determined by SEC-HPLC. |
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