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Biotinylated Human TIM-3/HAVCR2 Protein, Ultra Low Endotoxin (TIM-HM431B-UL) Summary
Product Description |
Recombinant Biotinylated Human TIM-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Arg200. |
Product Background |
Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human TIM-3/HAVCR2 Protein, Ultra Low Endotoxin (TIM-HM431B-UL) Specifications/Details
Protein |
TIM-3/HAVCR2 |
Synonyms |
CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser22-Arg200 |
Molecular Weight |
The protein has a predicted MW of 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Tim-3, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tim-3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human Tim-3, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tim-3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. |
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Biotinylated Human TIM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human TIM-3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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