Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-TNFR2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFR2, His Tag with the EC50 of 49.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu23-Asp257

Catalog: TNF-HM1R2B

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Product Description Recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257.

Product Background

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Product Category

Recombinant Protein

Target

TNFR2/CD120b/TNFRSF1B

Gene/Protein ID

P20333-1

Species

Human

Exact Sequence

Leu23-Asp257
Molecular Weight The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-TNFR2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFR2, His Tag with the EC50 of 49.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-TNFR2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFR2, His Tag with the EC50 of 49.7ng/ml determined by ELISA.
The purity of Biotinylated Human TNFR2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TNFR2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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