Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu23-Asp257
Catalog #TNF-HM1R2B
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Product Description
 Recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257.
Product Background
 Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Product Category
 Recombinant Protein
Target
 TNFR2/CD120b/TNFRSF1B
Synonyms
 CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu23-Asp257
Molecular Weight
 The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
Biotinylated Human TNFR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TNFR2 is greater than 95% as determined by SEC-HPLC.

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