Name: Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)
Brand: KactusBio
SKU: TNF-HM1R2B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B)

Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.

Biotinylated Human TNFR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human TNFR2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu23-Asp257

Catalog #TNF-HM1R2B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B) Summary

Product Description	Recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257.
Product Background	Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) (TNF-HM1R2B) Specifications/Details

Protein	TNFR2/CD120b/TNFRSF1B
Synonyms	CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B
Accession	P20333-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu23-Asp257
Molecular Weight	The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human TNFR2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
	Biotinylated Human TNFR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human TNFR2 is greater than 95% as determined by SEC-HPLC.