Biotinylated Human TNFSF15 Protein (FSF-HM415B)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu72-Leu251

Catalog: FSF-HM415B

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Product Description Recombinant Biotinylated Human TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus; It contains Leu72-Leu251.

Product Background

 TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Product Category

 Recombinant Protein

Target

 TNFSF15
Accession

Species

 Human

Amino Acid Range

 Leu72-Leu251
Molecular Weight The protein has a predicted MW of 23.38 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

 N-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human TNFSF15, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA.
Biotinylated Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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