Name: Biotinylated Human TNFSF15 Protein (FSF-HM415B)
Brand: KactusBio
SKU: FSF-HM415B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human TNFSF15 Protein (FSF-HM415B)

Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test).

Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 72.2ng/ml determined by ELISA.

Immobilized Biotinylated Human TNFSF15, His Avi Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA.

Immobilized Biotinylated Human TNFSF15, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA.

Biotinylated Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Biotinylated Human TNFSF15, His-Avi Tag with an affinity constant of 3.13 nM as determined in BLI assay (Gator).

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test).
Amino Acid Range: Leu72-Leu251
Other Available Proteins:

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Catalog #FSF-HM415B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human TNFSF15 Protein (FSF-HM415B) Summary

Product Description	Recombinant Biotinylated Human TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus; It contains Leu72-Leu251.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human TNFSF15 Protein (FSF-HM415B) Specifications/Details

Protein	TNFSF15
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession	O95150-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu72-Leu251
Molecular Weight	The protein has a predicted MW of 23.38 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test).
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test).
	Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 72.2ng/ml determined by ELISA.
	Immobilized Biotinylated Human TNFSF15, His Avi Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA.
	Immobilized Biotinylated Human TNFSF15, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA.
	Biotinylated Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Biotinylated Human TNFSF15, His-Avi Tag with an affinity constant of 3.13 nM as determined in BLI assay (Gator).