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Biotinylated Human TNFSF15 Protein, Ultra Low Endotoxin (FSF-HM415B-UL) Summary
Product Description |
Recombinant Biotinylated Human TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus; It contains Leu72-Leu251. |
Product Background |
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human TNFSF15 Protein, Ultra Low Endotoxin (FSF-HM415B-UL) Specifications/Details
Protein |
TNFSF15 |
Synonyms |
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu72-Leu251 |
Molecular Weight |
The protein has a predicted MW of 23.38 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test). The affinity constant of 1.51 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human TNFSF15, His-Avi Tag with an affinity constant of 1.51 nM as determined in SPR assay (Biacore S200). |
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Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test). |
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Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 72.2ng/ml determined by ELISA. |
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Immobilized Biotinylated Human TNFSF15, His Avi Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. |
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Immobilized Biotinylated Human TNFSF15, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA. |
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Biotinylated Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
Other Available Proteins:
Finding available proteins...
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