Name: Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) (FSF-HM416B)
Brand: KactusBio
SKU: FSF-HM416B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) (FSF-HM416B)

Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human TNFSF15 Trimer, His Tag with an affinity constant of 3.64 nM as determined in SPR assay (Biacore T200).

Immobilized Mouse DR3, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 ug/ml determined by ELISA (QC Test).

Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.34ug/ml determined by ELISA.

Biotinylated Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Flag
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Mouse DR3, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 ug/ml determined by ELISA (QC Test). The affinity constant of 3.64 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp91-Leu251

Catalog #FSF-HM416B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Asp91-Leu251.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein / Antibody discovery

Protein	TNFSF15 trimer
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession	O95150-1
Species	Human
Biotinylated	yes
Amino Acid Range	Asp91-Leu251
Molecular Weight	The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse DR3, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 ug/ml determined by ELISA (QC Test). The affinity constant of 3.64 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human TNFSF15 Trimer, His Tag with an affinity constant of 3.64 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse DR3, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 ug/ml determined by ELISA (QC Test).
	Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.34ug/ml determined by ELISA.
	Biotinylated Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.