Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM4R1B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.43ug/ml determined by ELISA. The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Pro34-Asn239

Catalog: TRL-HM4R1B

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Product Description Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Pro34-Asn239.
Product Category Recombinant Protein
Target TRAIL R1/DR4/TNFRSF10A
Synonyms DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365
Gene/Protein ID O00220
Species Human
Exact Sequence Pro34-Asn239
Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.43ug/ml determined by ELISA. The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200).
Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.43ug/ml determined by ELISA.
The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TRAIL R1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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