Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM4R1B)
Catalog: TRL-HM4R1B
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Product Description | Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Pro34-Asn239. |
Product Category | Recombinant Protein |
Target | TRAIL R1/DR4/TNFRSF10A |
Synonyms | DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365 |
Gene/Protein ID | O00220 |
Species | Human |
Exact Sequence | Pro34-Asn239 |
Molecular Weight | The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.43ug/ml determined by ELISA. The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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