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Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM4R1B) Summary
Product Description |
Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro34-Asn239. |
Product Background |
Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM4R1B) Specifications/Details
Protein |
TRAIL R1/DR4/TNFRSF10A |
Synonyms |
DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Pro34-Asn239 |
Molecular Weight |
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Human TRAIL R1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC. |
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