Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM4R1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA (QC Test). The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro34-Asn239
Catalog #TRL-HM4R1B
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Product Description
 Recombinant Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro34-Asn239.
Product Background
 Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells.
Product Category
 Recombinant Protein
Target
 TRAIL R1/DR4/TNFRSF10A
Synonyms
 DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Pro34-Asn239
Molecular Weight
 The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA (QC Test). The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200).
Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA (QC Test).
Biotinylated Human TRAIL R1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.

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