• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.135 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala56-His211
Catalog #TRL-HM4R4B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human TRAIL R4/TNFRSF10D Protein (TRL-HM4R4B) Summary

Product Description

 Recombinant Biotinylated Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala56-His211.

Product Background

 TNF-related apoptosis inducing ligand (TRAIL) is a potential antitumor protein known for its ability to selectively eliminate various types of tumor cells without exerting toxic effects in normal cells and tissues. TRAIL-R2/DR5 as well as TRAIL-R3/DcR1 and TRAIL-R4/DcR2 were significantly higher expressed in advanced tumour stages.

Biotinylated Human TRAIL R4/TNFRSF10D Protein (TRL-HM4R4B) Specifications/Details

Protein
 TRAIL R4/TNFRSF10D
Synonyms
 CD264; RSF10D; TRAILR4; DCR2; TRUNDD; TNFRSF10D
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala56-His211
Molecular Weight
 The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.135 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human TRAIL R4, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.135 nM as determined in SPR assay (Biacore T200). Biotinylated Human TRAIL R4, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.135 nM as determined in SPR assay (Biacore T200).
Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test).
Biotinylated Human TRAIL R4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human TRAIL R4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human TRAIL R4 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human TRAIL R4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...