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Biotinylated Human TRAIL R4/TNFRSF10D Protein (TRL-HM4R4B) Summary
Product Description |
Recombinant Biotinylated Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala56-His211. |
Product Background |
TNF-related apoptosis inducing ligand (TRAIL) is a potential antitumor protein known for its ability to selectively eliminate various types of tumor cells without exerting toxic effects in normal cells and tissues. TRAIL-R2/DR5 as well as TRAIL-R3/DcR1 and TRAIL-R4/DcR2 were significantly higher expressed in advanced tumour stages. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human TRAIL R4/TNFRSF10D Protein (TRL-HM4R4B) Specifications/Details
Protein |
TRAIL R4/TNFRSF10D |
Synonyms |
CD264; RSF10D; TRAILR4; DCR2; TRUNDD; TNFRSF10D |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala56-His211 |
Molecular Weight |
The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.135 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human TRAIL R4, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.135 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). |
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Biotinylated Human TRAIL R4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human TRAIL R4 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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