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Biotinylated Human Transferrin Protein (TFN-HM401B) Summary
Product Description |
Recombinant Biotinylated Human Transferrin Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Val20-Pro698. |
Product Background |
Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human Transferrin Protein (TFN-HM401B) Specifications/Details
Protein |
Transferrin |
Synonyms |
Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Val20-Pro698 |
Molecular Weight |
The protein has a predicted MW of 78.11 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Transferrin R, His Tag (Cat. TFR-HM102) at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 14.3ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Transferrin R, His Tag (Cat. TFR-HM102) at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 14.3ng/ml determined by ELISA. |
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Biotinylated Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Biotinylated Human Transferrin, His-Avi Tag on Streptavidin-Biosensor can bind Human Transferrin R, His Tag (Cat. TFR-HM102) with an affinity constant of 10.30 nM as determined in BLI assay . |
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The purity of Biotinylated Human Transferrin is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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