Biotinylated Human Transferrin Protein (TFN-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Transferrin R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val20-Pro698
Catalog #TFN-HM401B
Regular price $578.00
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Product Description

Recombinant Biotinylated Human Transferrin Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Val20-Pro698.

Product Background

Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Transferrin

Synonyms

Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Val20-Pro698

Molecular Weight

The protein has a predicted MW of 78.11 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Transferrin R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Transferrin R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 20.8ng/ml determined by ELISA. Immobilized Human Transferrin R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 20.8ng/ml determined by ELISA.
Biotinylated Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Transferrin is greater than 95% as determined by SEC-HPLC.

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