• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Transferrin R Apical Domain, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
  • Amino Acid Range: Lys189-Glu383
Catalog #TFR-HM4ADB
Price
Regular price $578.00
Regular price Sale price $578.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Transferrin R/CD71 Apical Domain Protein (TFR-HM4ADB) Summary

Product Description

 Recombinant Biotinylated Human Transferrin R/CD71 Apical Domain Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Lys189-Glu383.

Product Background

 The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human Transferrin R/CD71 Apical Domain Protein (TFR-HM4ADB) Specifications/Details

Protein
 Transferrin R/CD71
Synonyms
 TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Lys189-Glu383
Molecular Weight
 The protein has a predicted MW of 24.96 kDa. Due to glycosylation, the protein migrates to 32-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human Transferrin R Apical Domain, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Product Tag
 N-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human Transferrin R Apical Domain, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. Immobilized Biotinylated Human Transferrin R Apical Domain, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Biotinylated Human Transferrin R Apical Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Transferrin R Apical Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin R Apical Domain is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Transferrin R Apical Domain is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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