Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.73 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Cys89-Phe760
Catalog #TFR-HM401B
Regular price $577.50
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Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B) Summary

Product Description

Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.

Product Background

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B) Specifications/Details

Protein
Transferrin R/CD71
Synonyms
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Cys89-Phe760
Molecular Weight
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.73 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi
Expression System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200). Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Loaded Anti-Transferrin R Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 0.24 nM as determined in BLI assay (Gator® Prime). Loaded Anti-Transferrin R Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 0.24 nM as determined in BLI assay (Gator® Prime).
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