• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Cys89-Phe760
Catalog #TFR-HM401B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B) Summary

Product Description

 Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.

Product Background

 The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B) Specifications/Details

Protein
 Transferrin R/CD71
Synonyms
 TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Cys89-Phe760
Molecular Weight
 The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-95 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80ยฐC for 12 months as supplied from date of receipt.๏ผ›-80ยฐC for 3 months after reconstitution.๏ผ›Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200). Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin R is greater than 85% and the molecular weight of this protein is around 160-180 kDa as determined by SEC-MALS. The purity of Biotinylated Human Transferrin R is greater than 85% and the molecular weight of this protein is around 160-180 kDa as determined by SEC-MALS.
The purity of Biotinylated Human Transferrin R is greater than 85% as determined by SEC-HPLC. The purity of Biotinylated Human Transferrin R is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...