Name: Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
Brand: KactusBio
SKU: TFR-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)

Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).

Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).

Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.

Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.73 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Cys89-Phe760

Catalog #TFR-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
Product Background	The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Transferrin R/CD71
Synonyms	TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession	P02786
Species	Human
Biotinylated	yes
Amino Acid Range	Cys89-Phe760
Molecular Weight	The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.73 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
	Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).
	Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
	Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.