Product Description |
Recombinant Human TROP-2/TACSTD2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains His27-Thr274. |
Product Background |
Trop-2, also known as epithelial glycoprotein-1 antigen (EGP-1), is a protein that in humans is encoded by the TACSTD2 gene. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. |
Product Category |
Recombinant Protein |
Target |
TROP-2/TACSTD2 |
Synonyms |
EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His27-Thr274 |
Molecular Weight |
The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TROP-2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.2 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human TROP-2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.2 ng/ml determined by ELISA (QC Test). |
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Human TROP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Immobilized Human TROP-2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.2 ng/ml / 2.2 ng/ml / 2.3 ng/ml determined by ELISA. |
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The purity of Human TROP-2 is greater than 95% as determined by SEC-HPLC. |
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