Cynomolgus CD30/TNFRSF8 Protein (CD3-CM130)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus CD30, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD30 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala27-Pro394

Catalog: CD3-CM130

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Product Description	Recombinant Cynomolgus CD30/TNFRSF8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Pro394.
Product Background	The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
Product Category	Recombinant Protein

Target	CD30/TNFRSF8
Gene/Protein ID	A0A2K5VW07
Species	Cynomolgus
Exact Sequence	Ala27-Pro394
Molecular Weight	The protein has a predicted MW of 39.86 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus CD30, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD30 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus CD30, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD30 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
	The purity of Cynomolgus CD30 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.