Biotinylated Human HGF R/c-MET Protein (MET-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-HGF R Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu25-Thr932

Catalog: MET-HM401B

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Product Description Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu25-Thr932.
Product Category Recombinant Protein
Target HGF R/c-MET
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Gene/Protein ID P08581-1
Species Human
Exact Sequence Glu25-Thr932
Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-HGF R Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-HGF R Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25.7ng/ml determined by ELISA.
The purity of Biotinylated Human HGF R is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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