Biotinylated Human HGF R/c-MET Protein (MET-HM401B)
Catalog: MET-HM401B
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| Product Description | Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu25-Thr932. |
Product Background |
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. |
Product Category |
Recombinant Protein |
Target |
HGF R/c-MET |
| Synonyms | MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor |
Gene/Protein ID |
P08581-1 |
Species |
Human |
Exact Sequence |
Glu25-Thr932 |
| Molecular Weight | The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-HGF R Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-HGF R Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25.7ng/ml determined by ELISA. |
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The purity of Biotinylated Human HGF R is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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