• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HGF R, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
  • Amino Acid Range: Glu25-Thr932
Catalog #MET-HM401B
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human HGF R/c-MET Protein (MET-HM401B) Summary

Product Description

 Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu25-Thr932.

Product Background

 c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human HGF R/c-MET Protein (MET-HM401B) Specifications/Details

Protein
 HGF R/c-MET
Synonyms
 MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Glu25-Thr932
Molecular Weight
 The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human HGF R, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human HGF R, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. Immobilized Biotinylated Human HGF R, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Biotinylated Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HGF R is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HGF R is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...